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1 g of total RNA was reverse transcribed using Precision nanoscriptTM reverse transcriptase (Primerdesign, Southampton UK) and oligo dT primers (PrimerDesign, Southampton, UK)

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Muscle mass decreases, metabolism slows, and hormones that help regulate glucoselike DHEA, testosterone, and growth hormonebegin to drop

Evaluation the effect of phytosterol fraction of Chenopodium murale in comparison with Tacrolimus on mice induced atopic dermatitis

The membranes were blocked with 5% skimmed milk for 1 h at room temperature and then incubated with the desired primary antibodies and appropriate horseradish peroxidaseconjugated (HRP) secondary antibodies